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Biosearch Technologies Inc stellaris custom probe design software
Stellaris Custom Probe Design Software, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-designed+software/designer+fish+probe+rna+stellaris/pm41572818-71-39-44
Average 86 stars, based on 1 article reviews
stellaris custom probe design software - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Fluorescence In Situ Hybridization:

Article Title: Telocytes are a critical source of Wnts essential for hair follicle regeneration
Article Snippet: .. Custom probe libraries were designed utilizing the Stellaris FISH probe designer software based on sequence to hybridize with a desired coding RNA (Biosearch Technologies, Inc., Petaluma, CA) and were coupled to Cy5. ..

Article Title: mRNA quantification using single-molecule FISH in Drosophila embryos
Article Snippet: .. Wild type fruit flies (Bloomington Drosophila Stock Center, W 1118 ) Dry fine yeast (Lab Scientific Inc; FLY-8040–20F) Apple juice plate (see reagent setup) Clorox Ultra Germicidal Liquid Bleach (Fisher; 50371500) Heptane (Fisher Chemical; O3008–4) 20% Paraformaldehyde (Electron Microscopy Sciences; 15713) 100 % Methanol (Fisher Chemical; A412–4) Triton X-100 (Sigma; T8787–250 ml) Tween-20 (Sigma; P2287–500 ml) 10XPBS (PBS - Phosphate-Buffered Saline (10X) pH 7.4) (Thermo Fisher Scientific; AM 9624) Proteinase K (Sigma-Aldrich; 03 115879 001) Glycine (Sigma-Aldrich; G8790–1kg) 20XSSC (Saline-Sodium Citrate buffer) (Thermo Fisher Scientific; AM 9770) Deionized formamide (Amresco; 0606–100 ml) Ribonucleoside Vanadyl Complex (VRC) (New England Biolabs; S1402S) smFISH probe mix (Biosearch Technologies, Inc., commercially available and custom designed Stellaris® RNA FISH Probe Sets) Sheared salmon sperm DNA (ThermoFisher Scientific; AM9680) E.coli tRNA (Roche; 1010951001) Bovine Serum Albumin (BSA)(Roche; 10711454001) Dextran sulfate sodium salt (ACROS Organics; 433241000) DAPI (4’,6-Diamidine-2’-phenylindole Dihydrochloride) (Roche; 10 236 276 001) ProLong® Gold Antifade Reagent (Invitrogen (Molecular Probes); {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ) Vectashield (Vector Labs Inc, H-1000) Immersion oil (see Equipment setup , section Microscope ) .. list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 25oC incubator (ThermoFisher Scientific; FormaTM Enviromental Chambers) Embryo collection cage (Large: 8.75cm x 14.8cm) (Flystuff.com; 59–101) ( ).

Article Title: Detection of Nora virus in infected Drosophila melanogaster heads without evidence of ubiquitous brain infection by smRNA FISH.
Article Snippet: .. Following the third wash, the specimens were stored for several hours in PBST at 4°C until samples could be further processed. smRNA fluorescence in situ hybridization (smRNA FISH) probe design smRNA FISH probes targeting DmNV were designed with the Stellaris custom probe design software (Biosearch Technologies, Petaluma, CA; https://www.biosearchtech.com/products/rna-fish), following manufacturer instructions and recommendations. ..

Article Title: MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production.
Article Snippet: .. A pool of 48 oligonucleotides (listed in SI Appendix, Table S1), each labeled with a single Quasar 670 dye on the 3′ end and complementary to a different region of the target HIV-1 mRNA, were designed using custom FISH design software and manufactured by Biosearch Technologies. ..

Article Title: Detection of Nora virus in infected Drosophila melanogaster heads without evidence of ubiquitous brain infection by smRNA FISH
Article Snippet: .. smRNA FISH probes targeting DmNV were designed with the Stellaris custom probe design software (Biosearch Technologies, Petaluma, CA; https://www.biosearchtech.com/products/rna-fish ), following manufacturer instructions and recommendations. ..

Software:

Article Title: Telocytes are a critical source of Wnts essential for hair follicle regeneration
Article Snippet: .. Custom probe libraries were designed utilizing the Stellaris FISH probe designer software based on sequence to hybridize with a desired coding RNA (Biosearch Technologies, Inc., Petaluma, CA) and were coupled to Cy5. ..

Article Title: Detection of Nora virus in infected Drosophila melanogaster heads without evidence of ubiquitous brain infection by smRNA FISH.
Article Snippet: .. Following the third wash, the specimens were stored for several hours in PBST at 4°C until samples could be further processed. smRNA fluorescence in situ hybridization (smRNA FISH) probe design smRNA FISH probes targeting DmNV were designed with the Stellaris custom probe design software (Biosearch Technologies, Petaluma, CA; https://www.biosearchtech.com/products/rna-fish), following manufacturer instructions and recommendations. ..

Article Title: MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production.
Article Snippet: .. A pool of 48 oligonucleotides (listed in SI Appendix, Table S1), each labeled with a single Quasar 670 dye on the 3′ end and complementary to a different region of the target HIV-1 mRNA, were designed using custom FISH design software and manufactured by Biosearch Technologies. ..

Article Title: Detection of Nora virus in infected Drosophila melanogaster heads without evidence of ubiquitous brain infection by smRNA FISH
Article Snippet: .. smRNA FISH probes targeting DmNV were designed with the Stellaris custom probe design software (Biosearch Technologies, Petaluma, CA; https://www.biosearchtech.com/products/rna-fish ), following manufacturer instructions and recommendations. ..

Sequencing:

Article Title: Telocytes are a critical source of Wnts essential for hair follicle regeneration
Article Snippet: .. Custom probe libraries were designed utilizing the Stellaris FISH probe designer software based on sequence to hybridize with a desired coding RNA (Biosearch Technologies, Inc., Petaluma, CA) and were coupled to Cy5. ..

Electron Microscopy:

Article Title: mRNA quantification using single-molecule FISH in Drosophila embryos
Article Snippet: .. Wild type fruit flies (Bloomington Drosophila Stock Center, W 1118 ) Dry fine yeast (Lab Scientific Inc; FLY-8040–20F) Apple juice plate (see reagent setup) Clorox Ultra Germicidal Liquid Bleach (Fisher; 50371500) Heptane (Fisher Chemical; O3008–4) 20% Paraformaldehyde (Electron Microscopy Sciences; 15713) 100 % Methanol (Fisher Chemical; A412–4) Triton X-100 (Sigma; T8787–250 ml) Tween-20 (Sigma; P2287–500 ml) 10XPBS (PBS - Phosphate-Buffered Saline (10X) pH 7.4) (Thermo Fisher Scientific; AM 9624) Proteinase K (Sigma-Aldrich; 03 115879 001) Glycine (Sigma-Aldrich; G8790–1kg) 20XSSC (Saline-Sodium Citrate buffer) (Thermo Fisher Scientific; AM 9770) Deionized formamide (Amresco; 0606–100 ml) Ribonucleoside Vanadyl Complex (VRC) (New England Biolabs; S1402S) smFISH probe mix (Biosearch Technologies, Inc., commercially available and custom designed Stellaris® RNA FISH Probe Sets) Sheared salmon sperm DNA (ThermoFisher Scientific; AM9680) E.coli tRNA (Roche; 1010951001) Bovine Serum Albumin (BSA)(Roche; 10711454001) Dextran sulfate sodium salt (ACROS Organics; 433241000) DAPI (4’,6-Diamidine-2’-phenylindole Dihydrochloride) (Roche; 10 236 276 001) ProLong® Gold Antifade Reagent (Invitrogen (Molecular Probes); {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ) Vectashield (Vector Labs Inc, H-1000) Immersion oil (see Equipment setup , section Microscope ) .. list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 25oC incubator (ThermoFisher Scientific; FormaTM Enviromental Chambers) Embryo collection cage (Large: 8.75cm x 14.8cm) (Flystuff.com; 59–101) ( ).

Saline:

Article Title: mRNA quantification using single-molecule FISH in Drosophila embryos
Article Snippet: .. Wild type fruit flies (Bloomington Drosophila Stock Center, W 1118 ) Dry fine yeast (Lab Scientific Inc; FLY-8040–20F) Apple juice plate (see reagent setup) Clorox Ultra Germicidal Liquid Bleach (Fisher; 50371500) Heptane (Fisher Chemical; O3008–4) 20% Paraformaldehyde (Electron Microscopy Sciences; 15713) 100 % Methanol (Fisher Chemical; A412–4) Triton X-100 (Sigma; T8787–250 ml) Tween-20 (Sigma; P2287–500 ml) 10XPBS (PBS - Phosphate-Buffered Saline (10X) pH 7.4) (Thermo Fisher Scientific; AM 9624) Proteinase K (Sigma-Aldrich; 03 115879 001) Glycine (Sigma-Aldrich; G8790–1kg) 20XSSC (Saline-Sodium Citrate buffer) (Thermo Fisher Scientific; AM 9770) Deionized formamide (Amresco; 0606–100 ml) Ribonucleoside Vanadyl Complex (VRC) (New England Biolabs; S1402S) smFISH probe mix (Biosearch Technologies, Inc., commercially available and custom designed Stellaris® RNA FISH Probe Sets) Sheared salmon sperm DNA (ThermoFisher Scientific; AM9680) E.coli tRNA (Roche; 1010951001) Bovine Serum Albumin (BSA)(Roche; 10711454001) Dextran sulfate sodium salt (ACROS Organics; 433241000) DAPI (4’,6-Diamidine-2’-phenylindole Dihydrochloride) (Roche; 10 236 276 001) ProLong® Gold Antifade Reagent (Invitrogen (Molecular Probes); {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ) Vectashield (Vector Labs Inc, H-1000) Immersion oil (see Equipment setup , section Microscope ) .. list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 25oC incubator (ThermoFisher Scientific; FormaTM Enviromental Chambers) Embryo collection cage (Large: 8.75cm x 14.8cm) (Flystuff.com; 59–101) ( ).

Microscopy:

Article Title: mRNA quantification using single-molecule FISH in Drosophila embryos
Article Snippet: .. Wild type fruit flies (Bloomington Drosophila Stock Center, W 1118 ) Dry fine yeast (Lab Scientific Inc; FLY-8040–20F) Apple juice plate (see reagent setup) Clorox Ultra Germicidal Liquid Bleach (Fisher; 50371500) Heptane (Fisher Chemical; O3008–4) 20% Paraformaldehyde (Electron Microscopy Sciences; 15713) 100 % Methanol (Fisher Chemical; A412–4) Triton X-100 (Sigma; T8787–250 ml) Tween-20 (Sigma; P2287–500 ml) 10XPBS (PBS - Phosphate-Buffered Saline (10X) pH 7.4) (Thermo Fisher Scientific; AM 9624) Proteinase K (Sigma-Aldrich; 03 115879 001) Glycine (Sigma-Aldrich; G8790–1kg) 20XSSC (Saline-Sodium Citrate buffer) (Thermo Fisher Scientific; AM 9770) Deionized formamide (Amresco; 0606–100 ml) Ribonucleoside Vanadyl Complex (VRC) (New England Biolabs; S1402S) smFISH probe mix (Biosearch Technologies, Inc., commercially available and custom designed Stellaris® RNA FISH Probe Sets) Sheared salmon sperm DNA (ThermoFisher Scientific; AM9680) E.coli tRNA (Roche; 1010951001) Bovine Serum Albumin (BSA)(Roche; 10711454001) Dextran sulfate sodium salt (ACROS Organics; 433241000) DAPI (4’,6-Diamidine-2’-phenylindole Dihydrochloride) (Roche; 10 236 276 001) ProLong® Gold Antifade Reagent (Invitrogen (Molecular Probes); {"type":"entrez-protein","attrs":{"text":"P36934","term_id":"549428","term_text":"P36934"}} P36934 ) Vectashield (Vector Labs Inc, H-1000) Immersion oil (see Equipment setup , section Microscope ) .. list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 25oC incubator (ThermoFisher Scientific; FormaTM Enviromental Chambers) Embryo collection cage (Large: 8.75cm x 14.8cm) (Flystuff.com; 59–101) ( ).

Fluorescence:

Article Title: Detection of Nora virus in infected Drosophila melanogaster heads without evidence of ubiquitous brain infection by smRNA FISH.
Article Snippet: .. Following the third wash, the specimens were stored for several hours in PBST at 4°C until samples could be further processed. smRNA fluorescence in situ hybridization (smRNA FISH) probe design smRNA FISH probes targeting DmNV were designed with the Stellaris custom probe design software (Biosearch Technologies, Petaluma, CA; https://www.biosearchtech.com/products/rna-fish), following manufacturer instructions and recommendations. ..

In Situ Hybridization:

Article Title: Detection of Nora virus in infected Drosophila melanogaster heads without evidence of ubiquitous brain infection by smRNA FISH.
Article Snippet: .. Following the third wash, the specimens were stored for several hours in PBST at 4°C until samples could be further processed. smRNA fluorescence in situ hybridization (smRNA FISH) probe design smRNA FISH probes targeting DmNV were designed with the Stellaris custom probe design software (Biosearch Technologies, Petaluma, CA; https://www.biosearchtech.com/products/rna-fish), following manufacturer instructions and recommendations. ..

Labeling:

Article Title: MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production.
Article Snippet: .. A pool of 48 oligonucleotides (listed in SI Appendix, Table S1), each labeled with a single Quasar 670 dye on the 3′ end and complementary to a different region of the target HIV-1 mRNA, were designed using custom FISH design software and manufactured by Biosearch Technologies. ..



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Image Search Results


Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software Actitrack (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)

Journal: Brain Structure & Function

Article Title: Temporal progression of pathological features in an α-synuclein overexpression model of Parkinson’s disease

doi: 10.1007/s00429-025-02959-9

Figure Lengend Snippet: Open Field test in different experimental groups (control, sham, and α-syn) at one, two, and four months post-injection. Spontaneous global activity (a) , locomotion (b) , and mean velocity (c) were measured in an Open Field arena. Activity detection was fully automated using custom-designed software Actitrack (Panlab, Spain). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one-way ANOVA with post hoc corrections; n = 12 in each experimental group). (d) Temporal analysis assessing the progression of global activity. Data are presented as mean ± SEM. ***(time) p < 0.001; # (α-syn vs. control) p < 0.05 (multivariate linear regression analysis)

Article Snippet: The detection of global activity, locomotion and mean velocity was fully automated using the custom-designed software Actitrack (Panlab, Spain).

Techniques: Control, Injection, Activity Assay, Software